The efficacy of chloroquine treatment of Giardia duodenalis infection in calves
نویسندگان
چکیده
The purpose of the present study was to evaluate the effect of chloroquine treatment on cyst excretion in calves naturally infected with Giardia duodenalis. The calves were randomly assigned into two groups based on placebo (group I, n=7 untreated control calves) or treatment (group II, n=7 calves treated orally with 2.5 mg/kg chloroquine twice daily for five consecutive days). The G. duodenalis isolates were identified by molecular characterization with β-giardin nested PCR and gene sequence analysis as assemblage A3. Cyst excretion was determined on days 0, 3, 7 and 10, before and after treatment. Geometric means of the number of excreted cysts did not change significantly in the control group during the trial. The reduction in cyst excretion after chloroquine treatment was 99% on day 3 and 100% on days 7 and 10. Chloroquine treatment is most probatly practically applicable, relatively inexpensive and highly effective against giardiosis in calves.
منابع مشابه
A randomized, controlled, open-label trial evaluating the efficacy and safety of chloroquine in the treatment of giardiasis in children.
BACKGROUND Giardia duodenalis is among the commonest protozoan parasites in the intestinal tract of humans and may cause significant morbidity worldwide. Although there are several antigiardial agents, treatment failures have been commonly reported. OBJECTIVE To compare the efficacy and safety of chloroquine (CQ) versus metronidazole (MTZ) in the treatment of children with confirmed G duodena...
متن کاملMixed Giardia duodenalis assemblage A and E infections in calves.
A molecular epidemiological study was conducted on 100 dairy (499 calves) and 50 beef (333 calves) farms in Belgium to estimate the prevalence of different Giardia duodenalis assemblages in calves younger than 10 weeks of age. Positive samples from the epidemiological study and from a previous clinical study were selected and genotyped based on the amplification of the beta-giardin gene. To inv...
متن کاملPrevalence and genotyping of Giardia duodenalis among Arabian horses in Ahvaz, southwest of Iran
Giardia duodenalis is globally recognized as an important zoonotic intestinal protozoan parasite. So far, eight assemblages of G. duodenalis (A-H) have been identified. Substantial evidence suggests the zoonotic potential of assemblages A, B, and E in livestock. In this study, the genotype of Giardia duodenalis isolates was genetically identified by determining the sequence of ssu-rRNA gene and...
متن کاملA longitudinal study of Giardia duodenalis genotypes in dairy cows from birth to 2 years of age.
Fecal specimens were collected from 30 calves from birth to 24 months of age at a dairy farm in Maryland to determine the prevalence of Giardia duodenalis genotypes in cattle of different ages. Fecal samples were subjected to density gradient centrifugation to remove debris and concentrate cysts. Specimens were analyzed by immunofluorescence microscopy and polymerase chain reaction (PCR). All P...
متن کاملPrevalence of Giardia duodenalis assemblages in weaned cattle on cow-calf operations in the United States.
To determine the prevalence of Giardia duodenalis in weaned beef calves on cow-calf operations in the United States, fecal specimens were collected from 819 calves (6-18 months of age) from 49 operations. After cleaning and concentration procedures to maximize recovery of cysts from feces, DNA was extracted from each of the 819 specimens. The presence of G. duodenalis was determined by nested P...
متن کامل